HIMEDIA HiCrome 顯色培養基(/產色培養基/呈色培養基)

顯色培養基(/產色培養基/呈色培養基)
                                 ( Chromogenic / Fluorogenic Culture Media )


顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
        (一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形,
藉此直接觀察菌落顏色即可對菌種做出鑑定,
其反應的靈敏度和特異性大大優於傳統培養基。

顯色培養基通常為乾粉狀,容易儲存。
加蒸餾水溶解後,部分產品無須高壓滅菌,培養時間依具體培養基而定,
通常是18-24小時,比傳統時間顯著縮短。

HiMedia – M1574A_HiCrome™ EC 0157 H7 Agar Modified

Recommended for isolation and differentiation of Escherichia coli O157:H7 from food and environmental samples.
推薦用於從食品和環境樣品中分離和分化大腸桿菌O157:H7。
Principle and Interpretation
Escherichia coli O157:H7 belongs to the Enterohemorrhagic Escherichia coli (EHEC) group and it predominates as a food borne pathogen. E. coli O157: H7 was first recognized as a human pathogen in 1982 when two outbreaks of hemorrhagic colitis were associated with consumption of undercooked ground beef that has been contaminated with this organism (1). HiCrome™ EC O157:H7 Agar, Modified is a chromogenic medium recommended for the isolation and differentiation of E. coli O157:H7 from food and environmental samples. HiCrome™ ECO157:H7 Agar, Modified is based on the formulation described by Rappaport and Henigh (2). The medium contains sorbitol and a proprietary chromogenic mixture instead of lactose and indicator dyes respectively, as is conventionally used. The chromogenic substrate is specifically and selectively cleaved by E. coli O157: H7 resulting in a dark purple to magenta coloured moiety. E. coli gives bluish green coloured colonies. Tryptone and yeast extract provides carbonaceous, nitrogenous and growth nutrients. Bile salts mixture and SLS inhibits gram-positive organisms. Potassium tellurite selects the serogroups and inhibits Aeromonas species and Providencia species.
原理與解釋
大腸桿菌O157:H7屬於腸出血性大腸桿菌(EHEC)組,並且它主要作為食源性病原體。大腸桿菌O157:H7在1982年首次被認為是人類病原體,當時兩次爆發的出血性結腸炎與食用已被該生物體污染的未煮熟的碎牛肉有關(1)。 HiCrome™EC O157:H7 Agar,Modified是一種顯色培養基,推薦用於從食品和環境樣品中分離和分離大腸桿菌O157:H7。 HiCrome™ECO157:H7瓊脂,改性基於Rappaport和Henigh(2)描述的配方。該培養基含有山梨糖醇和專有的生色混合物,分別代替乳糖和指示劑染料,如常規使用的那樣。顯色底物被大腸桿菌O157:H7特異性和選擇性地切割,產生深紫色至品紅色部分。大腸桿菌產生藍綠色的菌落。 胰蛋白腖和酵母提取物提供碳質,含氮和生長營養素。膽汁鹽混合物和SLS抑制革蘭氏陽性生物。亞碲酸鉀選擇血清群並抑制氣單胞菌屬和普羅威登斯菌種。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
       相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌,
       HiMedia 顯色培養基產品 更多元,更豐富,品質優越。