HIMEDIA HiCrome 顯色培養基(/產色培養基/呈色培養基)

顯色培養基(/產色培養基/呈色培養基)
                                 ( Chromogenic / Fluorogenic Culture Media )


顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
        (一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形,
藉此直接觀察菌落顏色即可對菌種做出鑑定,
其反應的靈敏度和特異性大大優於傳統培養基。

顯色培養基通常為乾粉狀,容易儲存。
加蒸餾水溶解後,部分產品無須高壓滅菌,培養時間依具體培養基而定,
通常是18-24小時,比傳統時間顯著縮短。

HiMedia – M1850 HiColiform™ Broth, Modified

Recommended for the detection and confirmation of Escherichia coli and total coliforms from water samples, using a combination of chromogenic and fluorogenic substrates.
推薦用於檢測和確認水樣中的大腸桿菌和總大腸桿菌,使用生色和熒光底物的組合。
Principle and Interpretation
HiColiform™ Broth, Modified was designed for detection and confirmation of Escherichia coli and total coliforms from water samples using a combination of chromogenic and fluorogenic substrates. Escherichia coli can be distinguished from other coliforms by its unique ability to fluoresce in the presence of fluorogenic substrate (1, 2). The fluorogenic substrate is split by enzyme b-glucuronidase especially present in Escherichia coli. The reaction is indicated by the development of a blue fluorescence under UV light. The presence of total coliforms is indicated by blue-green colourations due to the cleavage of the chromogenic substrate. IPTG amplifies enzyme synthesis and increases the activity of b -galactosidase. Peptone provides essential growth nutrients and is useful for the simultaneous detection of indole production. The phosphate salts provide buffering action for rapid growth of coliforms. Sodium chloride helps to maintain the osmotic balance. Sodium lauryl sulphate makes the medium selective by inhibiting accompanying microflora, especially the gram-positive organisms.
原理與解釋
HiColiform™Broth,Modified用於使用生色和熒光底物的組合檢測和確認水樣中的大腸桿菌和總大腸桿菌。 通過其在熒光底物(1,2)存在下發熒光的獨特能力,可以將大腸桿菌與其他大腸桿菌區分開來。熒光底物被特別存在於大腸桿菌中的酶b-葡醣醛酸糖苷酶分開。通過在UV光下產生藍色熒光來指示反應。總大腸菌群的存在由藍綠色表示,因為它的裂解 髮色底物。 IPTG擴增酶合成並增加β-半乳糖苷酶的活性。 蛋白腖提供必需的生長營養素,可用於同時檢測吲哚的產生。磷酸鹽為大腸桿菌的快速生長提供緩衝作用。氯化鈉有助於維持滲透平衡。十二烷基硫酸鈉通過抑制伴隨的微生物群,特別是革蘭氏陽性生物,使培養基具有選擇性。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
       相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌,
       HiMedia 顯色培養基產品 更多元,更豐富,品質優越。