HM-PR_03-4MLT-Other media

HiMedia offers a broad range of Medias for Microbial Limit Testing (MLT) methods used in pharmaceutical Industries. The Microbial Limit Test is a primary and simple design to enumerate the number of microorganisms in terms of colony forming units (CFU) in a non-sterile product or raw material. The routine Microbial limit screening test consists of the total aerobic, yeast and mold counts as well as the test for objectionable microorganisms. The usual procedure involves, immersing the solution and then plate aliquots to determine the CFU/gram (or ml) of initial sample. The plating can be either pour plate, spread plate or the filtration of material and then placing the membrane filter on the surface of an agar plate. In case the product cannot be put into solution, then most probable Number Method can be employed where series of dilutions of the sample is carried out in a specified number of tubes containing suitable medium and under specified incubation conditions, will give an estimate of the microbial population of the product using most probable number tables. Another method i.e. Membrane Filtration is generally used when there are few expected colony forming units in the sample to be tested as it is a good method to test a large volume of liquid, but can only count up to approximately 100 CFU/membrane.
HiMedia提供廣泛的製藥行業微生物限度測試（MLT）方法介質。微生物限度測試是一種主要且簡單的設計，用於根據非無菌產品或原材料中的菌落形成單位（CFU）計算微生物的數量。常規微生物極限篩選試驗包括總需氧量，酵母菌和黴菌計數以及對有害微生物的檢測。通常的方法包括，浸泡溶液，然後用平板等分試樣測定初始樣品的CFU /克（或毫升）。電鍍可以是澆注板，擴散板或材料過濾，然後將膜過濾器放在瓊脂板的表面上。如果產品不能加入溶液，那麼最有可能的數量方法可以用於樣品的一系列稀釋液在指定數量的含有適當培養基的試管中並在規定的培養條件下進行，將給出微生物的估計值。使用最可能的數字表的產品的人口。另一種方法，即膜過濾通常在待測樣品中幾乎沒有預期的菌落形成單位時使用，因為它是測試大量液體的好方法，但只能計算到大約100CFU /膜。