HIMEDIA HiCrome 顯色培養基(/產色培養基/呈色培養基)

顯色培養基(/產色培養基/呈色培養基)
                                 ( Chromogenic / Fluorogenic Culture Media )


顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
        (一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形,
藉此直接觀察菌落顏色即可對菌種做出鑑定,
其反應的靈敏度和特異性大大優於傳統培養基。

顯色培養基通常為乾粉狀,容易儲存。
加蒸餾水溶解後,部分產品無須高壓滅菌,培養時間依具體培養基而定,
通常是18-24小時,比傳統時間顯著縮短。

HiMedia – M1417FandM1417 HiCrome™ Listeria Agar Base / Modified

Recommended as a selective and differential agar medium recommended for rapid and direct identification of Listeria species.
推薦用作選擇性和差異瓊脂培養基,推薦用於快速直接鑑定李斯特菌屬。
Principle and Interpretation
HiCrome™ Listeria Agar Base Modified medium is a modification of a medium first developed by Notermans et al. (3) and Mengaud et al. (2) for the detection of Listeria species from food stuffs. HiCrome™ Listeria Agar Base Modified allows growth of Listeria species and gives a presumptive identification of L. monocytogene within 24-48 hours after pre-enrichment. HiCrome™ Listeria Agar Base Modified (M1417) is based on rhamnose fermentation while HiCrome™ Listeria Agar Base (M1417F) is based on xylose fermentation. HiCrome™ Listeria Agar Base (M1417F) is in accordance with FDA BAM (1) where D-Xylose is the fermentable carbohydrate. This medium is based on the specific chromogenic detection of b-glucosidase activity and also sugar fermentation. Listeria species hydrolyse the purified chromogenic substrate in the medium forming bluish green coloured colonies. Since b-glucosidase activity is specific for Listeria species, other organisms cannot utilize the chromogenic substrate and therefore form colourless colonies. Differentiation between Listeria species is based on the property of rhamnose or xylose fermentation. The colonies of L. monocytogenes and L. innocua appear bluish green with a yellow halo (rhamnose positive) while the colonies of L. ivanovii appear blue without a yellow halo (rhamnose negative) in M1417. In case of M1417F, the colonies of L. ivanovii appear bluish green with yellow halo (xylose positive) while L. monocytogenes and L. innocua appear bluish green without a yellow halo (Xylose negative). Peptone special, peptone, yeast extract and HM extract provide nitrogenous, carbonaceous compounds, long chain amino acids, vitamin B complex and other essential growth nutrients. Rhamnose or xylose are the fermentable carbohydrates with phenol red as an indicator. Sodium chloride maintains the osmotic equilibrium. Lithium chloride and the added HiCrome™ Listeria Selective Supplement (FD181) inhibit growth of most gram positive bacteria, gram negative bacteria, yeasts and moulds.
原理與解釋
HiCrome™李斯特菌瓊脂基質改良培養基是由Notermans等人開發的培養基的改良物。 (3)和Mengaud等人。 (2)用於從食品中檢測李斯特菌屬物種。 HiCrome™李斯特菌瓊脂基因修飾允許李斯特菌屬的生長,並在預富集後24-48小時內對單核細胞增生症進行推定鑑定。 HiCrome™李斯特菌瓊脂基質修飾(M1417)基於鼠李糖發酵,而HiCrome™李斯特菌瓊脂基質(M1417F)基於木糖發酵。 HiCrome™李斯特菌瓊脂基質(M1417F)符合FDA BAM(1),其中D-木糖是可發酵的碳水化合物。該培養基基於β-葡糖苷酶活性的特定生色檢測以及糖發酵。 李斯特菌物種在培養基中水解純化的生色底物,形成藍綠色的菌落。由於β-葡糖苷酶活性對李斯特菌屬物種具有特異性,因此其他生物不能利用生色底物,因此形成無色菌落。李斯特菌屬物種之間的分化基於鼠李糖或木糖發酵的特性。單核細胞增生李斯特菌和無害李斯特菌的菌落呈現藍綠色,帶有黃色暈圈(鼠李糖陽性),而在伊斯特立諾威群島的菌落在M1417中呈現藍色而沒有黃色暈圈(鼠李糖陰性)。在M1417F的情況下,L。ivanovii的菌落呈現藍綠色,具有黃色暈(木糖陽性),而單核細胞增生李斯特菌和無害鏈球菌呈現藍綠色而沒有黃色暈(木糖陰性)。 蛋白腖,蛋白腖,酵母提取物和HM提取物提供含氮,含碳化合物,長鏈氨基酸,維生素B複合物和其他必需的生長營養素。鼠李糖或木糖是可發酵的碳水化合物,以酚紅為指示劑。氯化鈉保持滲透平衡。氯化鋰和添加的HiCrome™李斯特菌選擇性補充劑(FD181)可抑制大多數革蘭氏陽性菌,革蘭氏陰性菌,酵母菌和黴菌的生長。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
       相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌,
       HiMedia 顯色培養基產品 更多元,更豐富,品質優越。